N-Cadherin is a prospective cell surface marker of human mesenchymal stem cells that have high ability for cardiomyocyte differentiation

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• 作者：Hisako Ishimine ; Norio Yamakawa ; Mari Sasao ; Mika Tadokoro ; Daisuke Kami ; Shinji Komazaki ; Makoto Tokuhara ; Hitomi Takada ; Yoshimasa Ito ; Shinichiro Kuno ; Kotaro Yoshimura ; Akihiro Umezawa ; Hajime Ohgushi ; Makoto Asashima ; Akira Kurisaki
• 关键词：N-cadherin ; Flk-1 ; c-Kit ; Cardiomyocyte ; Mesenchymal stem cells
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2013
• 出版时间：6 September, 2013
• 年：2013
• 卷：438
• 期：4
• 页码：753-759
• 全文大小：1089 K

文摘

Mesenchymal stem cells (MSCs) are among the most promising sources of stem cells for regenerative medicine. However, the range of their differentiation ability is very limited. In this study, we explored prospective cell surface markers of human MSCs that readily differentiate into cardiomyocytes. When the cardiomyogenic differentiation potential and the expression of cell surface markers involved in heart development were analyzed using various immortalized human MSC lines, the MSCs with high expression of N-cadherin showed a higher probability of differentiation into beating cardiomyocytes. The differentiated cardiomyocytes expressed terminally differentiated cardiomyocyte-specific markers such as ¦Á-actinin, cardiac troponin T, and connexin-43. A similar correlation was observed with primary human MSCs derived from bone marrow and adipose tissue. Moreover, N-cadherin-positive MSCs isolated with N-cadherin antibody-conjugated magnetic beads showed an apparently higher ability to differentiate into cardiomyocytes than the N-cadherin-negative population. Quantitative polymerase chain reaction analyses demonstrated that the N-cadherin-positive population expressed significantly elevated levels of cardiomyogenic progenitor-specific transcription factors, including Nkx2.5, Hand1, and GATA4 mRNAs. Our results suggest that N-cadherin is a novel prospective cell surface marker of human MSCs that show a better ability for cardiomyocyte differentiation.