Identification of a HAD superfamily phosphatase, HdpA, involved in 1,3-dihydroxyacetone production during sugar catabolism in Corynebacterium glutamicum

详细信息    查看全文

• 作者：Toru Jojima ; Takafumi Igari ; Wataru Gunji ; Masako Suda ; Masayuki Inui ; Hideaki Yukawa ; ^{mmg-lab@rite.or.jp}
• 关键词：DHA ; 1 ; 3-dihydroxyacetone ; DHAP ; dihydroxyacetone phosphate ; HAD ; haloacid dehalogenase
• 刊名：FEBS Letters
• 出版年：2012
• 出版时间：30 November, 2012
• 年：2012
• 卷：586
• 期：23
• 页码：4228-4232
• 全文大小：329 K

文摘

Corynebacterium glutamicum produces 1,3-dihydroxyacetone (DHA) as metabolite of sugar catabolism but the responsible enzyme is yet to be identified. Using a transposon mutant library, the gene hdpA (cgR_2128) was shown to encode a haloacid dehalogenase superfamily member that catalyzes dephosphorylation of dihydroxyacetone phosphate to produce DHA. Inactivation of hdpA led to a drastic decrease in DHA production from each of glucose, fructose, and sucrose, indicating that HdpA is the main enzyme responsible for DHA production from sugars in C. glutamicum. Confirmation of DHA production via dihydroxyacetone phosphatase finally confirms a long-speculated route through which bacteria produce DHA.