RBC invasion and invasion-inhibition assays using free merozoites isolated after cold treatment of Babesia bovis in vitro culture
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- 作者：Takahiro Ishizaki ; Thillaiampalam Sivakumar ; Kyoko Hayashida ; Bumduuren Tuvshintulga ; Ikuo Igarashi ; Naoaki Yokoyama ; yokoyama@obihiro.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：Babesia bovis ; Cold treatment ; Invasion-inhibition assay ; Merozoite isolation ; RBC invasion
- 刊名：Experimental Parasitology
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：166
- 期：Complete
- 页码：10-15
- 全文大小：512 K
文摘
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Cold treatment induced the release of Babesia bovis merozoites from infected RBCs.
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Viability of the merozoites was higher than those liberated by high-voltage pulses.
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The merozoites released by cold treatment invaded the RBCs effectively.
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The merozoites were useful in RBC invasion and invasion-inhibition assays.
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The cold treatment can be used to obtain viable and invasion-competent merozoites.