Genetic, phenotypic and matrix-assisted laser desorption ionization time-of-flight mass spectrometry-based identification of anaerobic bacteria and determination of their antimicrobial susceptibility at a University Hospital in Japan

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• 作者：Tomoyuki Yunoki ^{tyunoki@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Yasufumi Matsumura ; ^{yazblood@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Satoshi Nakano ^{snakano@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Karin Kato ^{karin@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Go Hotta ^{hotta611@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Taro Noguchi ^{noguchit@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Masaki Yamamoto ^{masakiy@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Miki Nagao ^{mnagao@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Shunji Takakura ^{stakakr@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Satoshi Ichiyama ^{sichiyam@kuhp.kyoto-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Anaerobic bacteria ; Identification ; MALDI-TOF MS ; Antimicrobial susceptibility
• 刊名：Journal of Infection and Chemotherapy
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：22
• 期：5
• 页码：303-307
• 全文大小：229 K

文摘

The accuracies of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and the phenotypic method using VITEK 2 were compared to the accuracy of 16S rRNA sequence analysis for the identification of 170 clinically isolated anaerobes. The antimicrobial susceptibility of the isolates was also evaluated. Genetic analysis identified 21 Gram-positive species in 14 genera and 29 Gram-negative species in 11 genera. The most frequently isolated genera were Prevotella spp. (n = 46), Bacteroides spp. (n = 25) and Clostridium spp. (n = 25). MALDI-TOF MS correctly identified more isolates compared with VITEK 2 at the species (80 vs. 58%, respectively; p < 0.01) and genus (85 vs. 71%, respectively; p < 0.01) levels. More than 90% of the isolates of the three major genera identified (Prevotella, Bacteroides, and Clostridium species other than Clostridium difficile) were susceptible to beta-lactam/beta-lactamase inhibitor combinations, carbapenems, metronidazole and chloramphenicol. MALDI-TOF MS provided better identification results than VITEK2. Commonly used anti-anaerobic agents indicated that the isolates of the three most frequently identified anaerobic genera exhibited good antimicrobial susceptibility.