Regenerating gene I regulates interleukin-6 production in squamous esophageal cancer cells
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- 作者：Shuetsu Usami ; Satoru Motoyama ; Souichi Koyota ; Jingshu Wang ; Kaori Hayashi-Shibuya ; Kiyotomi Maruyama ; Naoko Takahashi ; Hajime Saito ; Yoshihiro Minamiya ; Shin Takasawa ; Jun-ichi Ogawa ; Toshihiro Sugi
- 关键词：REG I ; Interleukin-6 ; Cytokine ; Esophageal cancer
- 刊名：Biochemical and Biophysical Research Communications
- 出版年：2010
- 出版时间：29 January 2010
- 年：2010
- 卷：392
- 期：1
- 页码：4-8
- 全文大小：725 K
文摘
Regenerating gene (REG) I plays important roles in cancer cell biology. The purpose of this study was to determine whether REG I affects cytokine production in cancer cells. We transfected TE-5 and TE-9 squamous esophageal cancer cells with REG Iα and Iβ and examined its effects on cytokine expression. We found that transfecting TE-5 and TE-9 cells with REG I Iα and Iβ led to significantly increased expression of interleukin (IL)-6 mRNA and protein, but it had little or no effect on expression of IL-2, IL-4, IL-5, IL-10, IL-12, IL-13, IL-17A, interferon-γ, tumor necrosis factor-α, granulocyte-colony stimulating factor or transforming growth factor-β1. The elevated IL-6 expression seen in REG Iα transfectants was silenced by small interfering RNA-mediated knockdown. These finding suggest that REG I may act through IL-6 to exert effects on squamous esophageal cancer cell biology.