Dual Roles of Notch in Regulation of Apically Restricted Mitosis and Apicobasal Polarity of Neuroepithelial Cells

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• 作者：Shinya Ohata¹ ; Ryo Aoki¹ ; ² ; Shigeharu Kinoshita¹ ; ³ ; Masahiro Yamaguchi⁴ ; ⁵ ; Sachiko Tsuruoka-Kinoshita¹ ; Hideomi Tanaka¹ ; ⁶ ; Hironori Wada¹ ; ⁷ ; Shugo Watabe³ ; Takashi Tsuboi² ; Ichiro Masai⁴ ; Hitoshi Okamoto¹ ; ^{hitoshi@brain.riken.jp}
• 刊名：Neuron
• 出版年：2011
• 出版时间：27 January 2011
• 年：2011
• 卷：69
• 期：2
• 页码：215-230
• 全文大小：2916 K

文摘

Summary

How the mitosis of neuroepithelial stem cells is restricted to the apical ventricular area remains unclear. In zebrafish, the mosaic eyes^rw306 (moe/epb41l5^rw306) mutation disrupts the interaction between the putative adaptor protein Moe and the apicobasal polarity regulator Crumbs (Crb), and impairs the maintenance of neuroepithelial apicobasal polarity. While Crb interacts directly with Notch and inhibits its activity, Moe reverses this inhibition. In the moe^rw306 hindbrain, Notch activity is significantly reduced, and the number of cells that proliferate basally away from the apical area is increased. Surprisingly, activation of Notch in the moe^rw306 mutant rescues not only the basally localized proliferation but also the aberrant neuroepithelial apicobasal polarity. We present evidence that the CrbMoe complex and Notch play key roles in a positive feedback loop to maintain the apicobasal polarity and the apical-high basal-low gradient of Notch activity in neuroepithelial cells, both of which are essential for their apically restricted mitosis.