Up-regulation of hyaluronan receptors in Toxoplasma gondii-infected monocytic cells

详细信息    查看全文

• 作者：Akihiro Unno ; Katsuya Kitoh ; Yasuhiro Takashima
• 关键词：CD44 ; Hyaluronan ; ICAM-1 ; Monocyte ; Toxoplasma gondii
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2010
• 出版时间：1 January 2010
• 年：2010
• 卷：391
• 期：1
• 页码：477-480
• 全文大小：422 K

文摘

The apicomplexan, obligate intracellular parasite Toxoplasma gondii orally infects humans and animals. The parasites cross the intestinal epithelium, invade leukocytes in the general circulation and then disseminate into the peripheral organs. The mechanism of extravasation of the infected leukocytes, however, remains poorly understood. It is known that adhesion of leukocytes to extracellular matrix (ECM) is an important factor in extravasation, and CD44 and ICAM-1 on the leukocyte surface are known receptors for hyaluronan (HA), an ECM component. In this study, we demonstrated up-regulation of CD44 and ICAM-1 expression on the surface of T. gondii-infected human monocytic THP-1 cells and fresh isolated human monocyte. T. gondii-infected THP-1 cells adhered more efficiently to immobilized HA than did non-infected cells. T. gondii-infected monocytes in the general circulation might preferentially adhere to the ECM and migrate out from blood vessels, so transporting parasites into the peripheral organs.