RNA recognition mechanism of eukaryote tRNA (m⁷G46) methyltransferase (Trm8–Trm82 complex)

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• 作者：Keisuke Matsumoto ; Takashi Toyooka ; Chie Tomikawa ; Anna Ochi ; Yoshitaka Takano ; Naoyuki Takayanagi ; Yaeta Endo ; Hiroyuki Hori
• 关键词：RNA modification ; tRNA methyltransferase ; 7-Methylguanine ; Trm8– ; Trm82 ; TrmB ; Cell-free protein synthesis
• 刊名：FEBS Letters
• 出版年：2007
• 出版时间：17 April 2007
• 年：2007
• 卷：581
• 期：8
• 页码：1599-1604
• 全文大小：141 K

文摘

Yeast tRNA (m⁷G46) methyltransferase contains two protein subunits (Trm8 and Trm82). To address the RNA recognition mechanism of the Trm8–Trm82 complex, we investigated methyl acceptance activities of eight truncated yeast tRNA^Phe transcripts. Both the D-stem and T-stem structures were required for efficient methyl-transfer. To clarify the role of the D-stem structure, we tested four mutant transcripts, in which tertiary base pairs were disrupted. The tertiary base pairs were important but not essential for the methyl-transfer to yeast tRNA^Phe transcript, suggesting that these base pairs support the induced fit of the G46 base into the catalytic pocket.