- 作者：Hiroki Moriuchi M.D.* ; mkaki1@mac.com ; Yukio Kamohara M.D. ; Ph.D.* ; Susumu Eguchi M.D. ; Ph.D.* ; Weili Gu M.D. ; Ph.D.* ; Hikaru Fujioka M.D. ; Ph.D.* ; Takao Yamamoto M.D. ; Ph.D.* ; Yoshitsugu Tajima M.D. ; Ph.D.* ; Takashi Kanematsu M.D. ; Ph.D.* ; Takehiko Koji Ph.D.† ;
- 关键词：FK506 ; apoptosis ; liver transplantation ; Fas ; Fas ligand ; Bax ; Bcl-2
- 刊名：Journal of Surgical Research
- 出版年：2011
- 出版时间：1 May 2011
- 年：2011
- 卷：167
- 期：1
- 页码：131-139
- 全文大小：1763 K
Background
The current study investigated whether FK506 (FK) regulates the apoptotic systems in allografted rat liver and the contribution of Fas/Fas-ligand system and Bcl-2 family during acute rejection.Materials and Methods
The recipients were divided into three groups, the allo, the allo-FK, and the syn group. Rats were euthanized 1, 3, 5, and 7 d after OLT. Apoptotic activity was explored using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay. The expression of Fas/Fas-ligand and Bcl-2/Bax in the grafted livers was investigated by Western blotting and immunohistochemistry.
Results
The apoptotic index (AI) of hepatocytes in the allo-FK group was less than that in the allo group. Fas in the allo group was more intense than that in the allo-FK group in the periportal areas on day 1 and 3, while Bcl-2 in the allo group was less intense than that in the allo-FK group in the pericaval areas at all time-points after OLT.
Conclusion
FK provides beneficial antiapoptotic effects on hepatocytes in the grafted rat livers through both the down-regulation of Fas expression in the periportal areas and the up-regulation of Bcl-2 expression in the pericaval areas.