Marked increases in hippocampal neuron indoleamine 2, 3-dioxygenase via IFN-γ-independent pathway following transient global ischemia in mouse
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- 作者：Masato Hoshi ; Kuniaki Saito ; Yuki Murakami ; Ayako Taguchi ; Hidetsugu Fujigaki ; Ryo Tanaka ; Masao Takemura ; Hiroyasu Ito ; Akira Hara ; Mitsuru Seishima
- 关键词：Indoleamine 2 ; 3-Dioxygenase ; IFN-gamma KO mouse ; Immunohistochemistry
- 刊名：Neuroscience Research
- 出版年：2009
- 出版时间：March 2009
- 年：2009
- 卷：63
- 期：3
- 页码：194-198
- 全文大小：954 K
文摘
Indoleamine 2, 3-dioxygenase (IDO), which catabolizes L-tryptophan (L-TRP) to L-kynurenine (L-KYN), is an immunoregulatory factor that is up-regulated via an interferon-gamma (IFN-γ)-dependent and/or -independent mechanism. In this study, we investigated the localization of IDO and whether induction of IDO expression is an IFN-γ-dependent and/or -independent mechanism in the CNS after cerebral ischemia. The expressions of IDO protein and mRNA were investigated at different time points following cerebral ischemia using immunohistochemistry, immunofluorescence and RT-PCR. Hippocampal neuron IDO mRNA and immunohistochemical staining were significantly up-regulated 72 h after transient global ischemia. Although IFN-γ is a dominant inducer of IDO, hippocampal neuron IDO was clearly up-regulated in IFN-γ KO mice. In summary, this is the first finding that up-regulation of IDO in hippocampal neurons after transient global ischemia occurs via INF-γ-independent mechanisms.