Production optimization, preliminary characterization and bioactivity of exopolysaccharides from Incutis tamaricis (Pat.) Fiasson & Niemela

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• 作者：Jian-Qiang Zheng^a ; ^b ; Xiao-Jing Mao^c ; Lu-Jing Geng^b ; Gong-Ming Yang^a ; Chun-Ping Xu^b ; ^{c.p.xu@zzuli.edu.cn" class="auth_mail} ; ^{xuchunping05@163.com" class="auth_mail}
• 关键词：Bioactivity ; Chromatography ; Exopolysaccharides ; Fermentation ; Inocutis tamaricis
• 刊名：Journal of the Taiwan Institute of Chemical Engineers
• 出版年：May, 2014
• 年：2014
• 卷：45
• 期：3
• 页码：725-733
• 全文大小：907 K

文摘

The aim of this work was to optimize the submerged culture medium for exopolysaccharides (EPS) production from Inocutis tamaricis (Pat.) Fiasson & Niemel盲. The molecular characterization and the evaluation of antioxidant activity and antitumor activity in vitro of EPS were carried out as well. The one-factor-at-a-time method was adopted to investigate the optimal initial pH and temperature as 8.0 and 25 掳C, respectively. The optimal culture condition was obtained as follows: sucrose 64.09 g/L and tryptone 5.24 g/L by central composite design (CCD). Under optimal culture condition, the maximum EPS concentration in shake flask was 5.89 g/L. Two groups of EPSs (designated as Fr-I and Fr-II) were obtained from the culture filtrates by size exclusion chromatography (SEC), and their molecular characteristics were examined by a multiangle laser-light scattering (MALLS) and refractive index (RI) detector system. The weight-average molar masses of the Fr-I and Fr-II of EPS were determined to be 8.948 脳 10⁴ and 6.421 脳 10⁴ g/mol, respectively. The SEC/MALLS analysis revealed that the molecular shapes of the Fr-I and Fr-II were the rigid sphere suspected to be the aggregates of complex polysaccharides. Furthermore, Fr-II was exhibited higher antioxidant and antitumour activities than Fr-I. Finally, FT-IR spectroscopy was used for obtaining vibrational spectra of the Fr-II EPS and the obvious absorption peaks at 916.5 and 884.0 cm^鈭? revealed the co-existence of 伪 and 尾 configuration. The carbohydrate composition analysis revealed Fr-II EPS was mainly composed of mannose, glucose and talose.