Characterization and comparison of ATF and TFF in stirred bioreactors for continuous mammalian cell culture processes
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- 作者：Daniel J. Karsta ; Elisa Serraa ; Thomas K. Villigera ; Miroslav Soosa ; b ; miroslav.soos@vscht.cz" class="auth_mail" title="E-mail the corresponding author ; Massimo Morbidellia ; massimo.morbidelli@chem.ethz.ch" class="auth_mail" title="E-mail the corresponding author
- 关键词：2-AB ; 2-aminobenzamide ; AMM ; ammonia ; ATF ; alternating tangential flow ; CCC ; critical coagulation concentration ; GLC ; glucose ; HF ; hollow fiber ; LAC ; lactate ; LMH ; cross flow rate per unit area and volume ; PES ; polyether sulfone ; PMMA ; poly(methyl methacrylate) ; RT ; rushton turbine (impeller) ; RV ; reactor volume ; SALS ; small angle light scattering ; TFF ; tangential flow filtration ; TMP ; transmembrane Pressure ; VCD ; viable cell density
- 刊名：Biochemical Engineering Journal
- 出版年：2016
- 出版时间：15 June 2016
- 年：2016
- 卷：110
- 期：Complete
- 页码：17-26
- 全文大小：1100 K
文摘
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Two stirred tank perfusion setups (ATF and TFF) were physically characterized.
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Chosen operating parameters were confirmed by stable culture of CHO cells at 20, 40 and 60 × 106 cells/mL.
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Increased product retention was found in the TFF compared to the ATF.
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Integration of physical and cell culture characterization is a reliable approach to design stirred tank perfusion cultures