A simple LC/MRM-MS-based method to quantify free linker-payload in antibody-drug conjugate preparations
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- 作者：Wesley Zmolek ; Stefanie Bañ ; as ; Robyn M. Barfield ; David Rabuka ; Penelope M. Drake ; penelope.drake@catalent.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：ADC ; antibody drug conjugate ; MRM ; multiple-reaction monitoring ; LLOQ ; lower limit of quantification ; DMSO ; dimethyl sulfoxide ; ACN ; acetonitrile ; RP-HPLC ; reversed-phase high pressure liquid chromatography ; TFF ; tangential flow filtration
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：1 October 2016
- 年：2016
- 卷：1032
- 期：Complete
- 页码：144-148
- 全文大小：381 K
文摘
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A simple MRM-based method to quantify free linker-payload in an ADC is described.
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The approach has an LLOQ of 10 nM and requires no special laboratory equipment.
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The method was used to monitor free linker-payload removal during ADC manufacturing.