Hemagglutinin and neuraminidase containing virus-like particles produced in HEK-293 suspension culture: An effective influenza vaccine candidate
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- 作者：Alina Venereo-Sancheza ; b ; Renald Gilbertb ; Melanie Simoneaub ; Antoine Caronb ; Parminder Chahalb ; Wangxue Chenc ; Sven Ansorgeb ; Xuguang Lid ; Olivier Henrya ; Amine Kamene ; amine.kamen@mcgill.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：Influenza vaccine ; Virus-like particles ; Stable cell line ; HEK-293 ; Quantification ; Bioreactor production ; Tangential flow filtration
- 刊名：Vaccine
- 出版年：2016
- 出版时间：17 June 2016
- 年：2016
- 卷：34
- 期：29
- 页码：3371-3380
- 全文大小：2465 K
文摘
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Development of stable cell line expressing hemagglutinin and neuraminidase (293HA-NA).
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Gag HIV-1 protein is more efficient than M1 to induce VLPs production in 293HA-NA cells.
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Influenza VLPs production using Gag as scaffold was evaluated in a 3-L bioreactor and purified.
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Characterization of VLPs showed typical size and morphology of HIV-1 immature particles.
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The immunogenicity and protective efficacy of the VLPs was demonstrated in mice.