Targeted mutagenesis of a fatty acid Δ⁶-desaturase from Mucor rouxii: Role of amino acid residues adjacent to histidine-rich motif II

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• 作者：Sutthicha Na-Ranong ; Kobkul Laoteng ; Prasat Kittakoop ; Morakot Tanticharoen and Supapon Cheevadhanarak
• 关键词：Δ ; ⁶-Desaturase ; Mucor rouxii ; γ ; -Linolenic acid ; Histidine-rich motif ; Site-directed mutagenesis ; Substrate recognition
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2006
• 出版时间：27 January 2006
• 年：2006
• 卷：339
• 期：4
• 页码：1029-1034
• 全文大小：317 K

文摘

The amino acid residues serine at position 213 (S213) and lysine at position 218 (K218), which are present in close proximity to the histidine-rich motif II of Mucor rouxii fatty acid Δ⁶-desaturase isoform II, were targeted for studying structure–function relationships using site-directed mutagenesis. The mutants were functionally characterized in a heterologous host, Saccharomyces cerevisiae. Substrate specificity and preference studies revealed that S213 and K218 are involved in substrate recognition. K218 plays a role in substrate preference by involvement in the binding of substrates, particularly C15–C18 monoene fatty acids. Modification of the M. rouxii Δ⁶-desaturase therefore has potential in specifically altering substrate utilization for production of desired fatty acids.