A sensitive and high throughput TaqMan-based reverse transcription quantitative polymerase chain reaction assay efficiently discriminates ALK rearrangement from overexpression for lung cancer FFPE specimens
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- 作者：Jrhau Lunga ; 1 ; Yu-Ching Linb ; c ; d ; 1 ; Ming-Szu Hungb ; c ; d ; Yuan Yuan Jiangb ; Kuan-Der Leec ; e ; Paul Yann Linf ; Ying Huang Tsaib ; g ; chestmed@cgmh.org.tw" class="auth_mail" title="E-mail the corresponding author
- 关键词：Lung cancer ; Anaplastic lymphoma kinase ; Real time PCR ; FISH ; Immunohistochemisty
- 刊名：Lung Cancer
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：94
- 期：Complete
- 页码：114-120
- 全文大小：1289 K
文摘
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A sensitive and high throughput TaqMan based ALK qPCR assay was established.
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High concordance between qPCR, FISH and IHC based ALK tests.
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The qPCR assay can efficiently discriminate ALK rearrangement from overexpression.
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The qPCR assay helps clarify the discrepancies between ALK FISH and IHC results.