Development of RT-qPCR assays for the specific identification of two major genotypes of avian infectious bronchitis virus
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- 作者：Ana Marandinoa ; Gonzalo Tomá ; sa ; Martí ; n Herná ; ndeza ; Yanina Panzeraa ; Marí ; a Isabel Craigb ; Ariel Vagnozzib ; Federico Verac ; Claudia Techeraa ; Sofí ; a Greccoa ; Alejandro Bandad ; Diego Herná ; ndeza ; Ruben Pé ; reza ; rperez@fcien.edu.uy" class="auth_mail" title="E-mail the corresponding author
- 关键词：Infectious bronchitis ; IBV ; RT-qPCR ; Genotype
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：235
- 期：Complete
- 页码：21-25
- 全文大小：543 K
文摘
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RT-qPCR assays were developed for the detection of two major IBV genotypes.
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The assays use TaqMan probes that target sequences in the spike glycoprotein gene.
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The RT-qPCR assays were specific, sensitive and reproducible.
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A large number of clinical samples can be easily screened.
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The assays are expected to improve our understanding of IBV epidemiology.