Detection of Strawberry necrotic shock virus using conventional and TaqMan® quantitative RT-PCR
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- 作者：Thanuja Thekke Veetila ; 1 ; Thien Hoa ; 1 ; Catalina Moyerb ; Vance M. Whitakerb ; Ioannis E. Tzanetakisa ; itzaneta@uark.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Strawberry necrotic shock virus ; Virus detection ; Taqman qPCR
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：235
- 期：Complete
- 页码：176-181
- 全文大小：1186 K
文摘
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New universal detection protocols have been developed for Strawberry necrotic shock virus.
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Conventional PCR can detect as low as 4 pg/μl of virus RNA; qPCR 40fg/μl of virus RNA.
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The assays have been successfully used on field material.
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This research has major implications for certification programs around the world.