Quantitative detection of pork in commercial meat products by TaqMan® real-time PCR assay targeting the mitochondrial D-loop region
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- 作者：Miju Kim ; Insuk Yoo ; Shin-Young Lee ; Yeun Hong ; Hae-Yeong Kim ; hykim@khu.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：TaqMan real-time PCR ; Pork ; Mitochondrial D-loop region ; Meat products ; Quantification
- 刊名：Food Chemistry
- 出版年：2016
- 出版时间：1 November 2016
- 年：2016
- 卷：210
- 期：Complete
- 页码：102-106
- 全文大小：554 K
文摘
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Pork-specific primers and probe were developed using the mitochondrial D-loop region.
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TaqMan® real-time PCR was established for quantitative PCR detection of pork meat.
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The detection limit of this assay was 0.1 pg of DNA from heat-treated pork meat.
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Commercial meat products were used to verify the applicability of this method.