Optimization of methyl jasmonate and β-cyclodextrin for enhanced production of taraxerol and taraxasterol in (Taraxacum officinale Weber) cultures
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- 作者：Kiran Sharma ; Rasheeduz Zafar ; Rasheedzafar@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：β-Cyclodextrin (CD) ; Methyl jasmonate (MJ) ; RP-HPLC ; Taraxacum officinale (TO) ; Taraxerol (TA) ; Taraxasterol (TX)
- 刊名：Plant Physiology and Biochemistry
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：103
- 期：Complete
- 页码：24-30
- 全文大小：832 K
文摘
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Taraxacum officinale commonly known as dandelion contains compounds Taraxerol (TA) and Taraxasterol (TX) in its roots.
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Both these compounds are present in plant in very small amount and are reported to be anticancer.
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Abiotic elicitors Methyl Jasmonate (MJ) and β-Cyclodextrin (CD) were added to the root callus suspension cultures of Dandelion.
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Central composite design (CCD) was used to optimize the concentration of MJ and CD to obtain improved yield of TA and TX.
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Quantification of TA and TX from the elicitor added cultures was done by High Performance Liquid Chromatography (HPLC).