Partitioning and purification of menadione induced NAD(P)H oxidase from Phanerochaete chrysosporium in aqueous two-phase systems
详细信息 查看全文
- 作者：Burcu Tongul burcutongul@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Berna Kavakcıoglu berna.kavakcioglu@deu.edu.tr" class="auth_mail" title="E-mail the corresponding author ; Leman Tarhan ; leman.tarhan@deu.edu.tr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Aqueous two-phase systems ; Polymer/salt system ; NAD(P)H oxidase ; Partitioning ; Purification
- 刊名：Separation and Purification Technology
- 出版年：2016
- 出版时间：11 May 2016
- 年：2016
- 卷：163
- 期：Complete
- 页码：275-281
- 全文大小：581 K
文摘
- •
Different ATPSs were investigated for the purification of NAD(P)H oxidase.
- •
In the best PEG 2000 18%–K2HPO4 13%–NaCl 5% (pH 7.0) system, PFb was determined as 8.331.
- •
For this system, Rb was found as 87.644%.
- •
It was found that NAD(P)H oxidase was a homodimer with the molecular weight of 116 kDa.