Alternating isocratic and step gradient elution high-speed counter-current chromatography for the isolation of minor phenolics from Ormocarpum kirkii bark

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• 作者：Eutrophe Le Doux Kamto^a ; ^b ; Tatiane S.C. Carvalho^b ; José ; phine Ngo Mbing^a ; Marie C.N. Matene^a ; Dieudonné ; E. Pegnyemb^a ; Gilda G. Leitã ; o^b ; ^{ggleitao@yahoo.com.br} ; ^{ggleitao@nppn.ufrj.br}
• 关键词：Ormocarpum kirkii ; Fabaceae ; Flavones ; Biflavonoids ; Biscoumarins ; Gradient elution CCC
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：13 January 2017
• 年：2017
• 卷：1480
• 期：Complete
• 页码：50-61
• 全文大小：2190 K
• 卷排序：1480

文摘

Two new flavones and one biflavanone glycosides were isolated from Ormocarpum kirkii by HSCCC. Application of several solvent systems afforded 14 compounds with different polarities. Structures of compounds were identified by HPLC-UV, ESI-MS, 1D and 2D NMR. The present study shows that HSCCC is a useful and fast method for natural product isolation.