Chemical derivatization for enhancing sensitivity during LC/ESI-MS/MS quantification of steroids in biological samples: a review
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- 作者：Tatsuya Higashi ; higashi@rs.tus.ac.jp" class="auth_mail" title="E-mail the corresponding author ; Shoujiro Ogawa
- 关键词：1 ; 25(OH)2D2 ; 1α ; 25-dihydroxyvitamin D2 ; 1 ; 25(OH)2D3 ; 1α ; 25-dihydroxyvitamin D3 ; 17(OH)P4 ; 17α-hydroxyprogesterone ; 17(OH)P5 ; 17α-hydroxypregnenolone ; 24 ; 25(OH)2D3 ; 24R ; 25-dihydroxyvitamin D3 ; 25(OH)D2 ; 25-hydroxyvitamin D2 ; 25(OH)D3 ; 25-hydroxyvitamin D3 ; 3α ; 5α-Adiol ; 3α ; 5α-androstanediol ; 4 ; 25(OH)2D3 ; 4β ; 25-dihydroxyvitamin D3 ; AD ; androstenedione ; AP ; allopregnanolone ; APA ; aldosterone-producing adenoma ; APCI ; atmospheric pressure chemical ionization ; BPH ; benign prostate hyperplasia ; CAH ; con
- 刊名：Journal of Steroid Biochemistry and Molecular Biology
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：162
- 期：Complete
- 页码：57-69
- 全文大小：686 K
文摘
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LC/ESI–MS/MS has been widely used for the quantification of endogenous steroids.
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However, the ESI efficiency and fragmentation behavior of some steroids are poor.
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Chemical derivatization is effective for enhancing detectability of steroids.
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This article reviews chemical derivatization for LC/ESI–MS/MS of steroids.
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Stable isotope-coded derivatization for the steroid analysis is also described.