Homocysteine and copper induce cellular apoptosis via caspase activation and nuclear translocation of apoptosis-inducing factor in neuronal cell line SH-SY5Y

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• 作者：Yoshimasa Hirashima ; So Seshimo ; Yoshika Fujiki ; Masaaki Okabe ; Kazuo Nishiyama ; Mitsuharu Matsumoto ; Hiroaki Kanouchi ; Tatsuzo Oka
• 关键词：Homocysteine ; Copper ; Apoptosis ; Reactive oxygen species ; Apoptosis-inducing factor
• 刊名：Neuroscience Research
• 出版年：2010
• 出版时间：August 2010
• 年：2010
• 卷：67
• 期：4
• 页码：300-306
• 全文大小：2847 K

文摘

Hyperhomocysteinemia has been implicated in dementia and neurodegenerative disease. Physiological homocysteine concentrations did not result in apoptosis in SH-SY5Y cells in the present study. The apoptosis was recognized in millimolar level of homocysteine. However, SH-SY5Y cell death was observed following exposure to micromolar level of homocysteine in combination with copper. Exposure to 250 μM homocysteine and 10 μM CuCl₂ for one day decreased cell viability by 40 % . Homocysteine and copper caused apoptosis, because hallmarks of apoptosis were recognized, such as loss of mitochondrial membrane potential, TUNEL-positive cells, release of cytochrome c from mitochondria, and caspase-3 activation, but not nucleosomal DNA fragmentation. Homocysteine and copper generated the intracellular reactive oxygen species, and homocysteine and copper-induced apoptosis was due to an accumulation of intracellular reactive oxygen species, which was inhibited by catalase. Pan-caspase inhibitor, z-VAD-fmk, could not completely inhibited homocysteine and copper-induced cell death. Homocysteine and copper also caused the nuclear translocation of apoptosis-inducing factor. These results suggested that homocysteine and copper induced not only caspase-dependent apoptosis but also caspase-independent apoptosis-inducing factor related apoptosis.