Flavanone and isoflavone glucosylation by non-Leloir glycosyltransferases
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- 作者：Heike Overwina ; Victor Wrayb ; Michael Seegerc ; Silvia Sepú ; lveda-Bozad ; Bernd Hofera ; bernd.hofer@helmholtz-hzi.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Ams-Np ; amylosucrase from Neisseria polysaccharea ; AS ; amylosucrase ; Glc ; glucose ; COSY ; correlation spectroscopy ; GS ; glucansucrase ; GtfR-So ; glucansucrase from Streptococcus oralis ; HPLC ; high-performance liquid chromatography ; HRMS ; high resolution mass spectrometry ; NMR ; nuclear magnetic resonance ; OD600 ; optical density at 600 ; nm ; ROESY ; rotating-frame Overhauser effect spectroscopy ; Tax ; taxifolin ; TLC ; thin-layer chromatography ; UGT ; UDP-glycoside-dependent glycosyltransferase
- 刊名：Journal of Biotechnology
- 出版年：2016
- 出版时间：10 September 2016
- 年：2016
- 卷：233
- 期：Complete
- 页码：121-128
- 全文大小：1071 K
文摘
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Amylo- and glucansucrases catalyze the O-glucosylation of isoflavones.
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Isoflavones were α-glucosylated in the A ring at O7.
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With a given substituent pattern, the flavonoid class was critical for catalysis.
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The flavanone taxifolin was α-glucosylated at the para position of the B ring.
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Typically multiple products were obtained, differing in glucose content.