Enhanced osteogenic differentiation of stem cells via microfluidics synthesized nanoparticles

详细信息    查看全文

• 作者：Mohammad Mahdi Hasani-Sadrabadi ; PhD^a ; ^b ; ^c ; Sana Pour Hajrezaei ; MSc^b ; Shahriar Hojjati Emami ; PhD^b ; Ghasem Bahlakeh ; PhD^b ; Leila Daneshmandi ; MSc^d ; Erfan Dashtimoghadam ; PhD^e ; Ehsan Seyedjafari ; PhD^f ; Karl I. Jacob ; PhD^a ; ^{karl.jacob@mse.gatech.edu" class="auth_mail" title="E-mail the corresponding author} ; Lobat Tayebi ; PhD^e ; ^g ; ^{lobat.tayebi@marquette.edu" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Polymeric nanoparticles ; Microfluidics platform ; Drug delivery ; Osteogenic differentiation
• 刊名：Nanomedicine: Nanotechnology, Biology and Medicine
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：11
• 期：7
• 页码：1809-1819
• 全文大小：1655 K

文摘

Advancement of bone tissue engineering as an alternative for bone regeneration has attracted significant interest due to its potential in reducing the costs and surgical trauma affiliated with the effective treatment of bone defects. We have improved the conventional approach of producing polymeric nanoparticles, as one of the most promising choices for drug delivery systems, using a microfluidics platform, thus further improving our control over osteogenic differentiation of mesenchymal stem cells. Molecular dynamics simulations were carried out for theoretical understanding of our experiments in order to get a more detailed molecular-scale insight into the drug–carrier interactions. In this work, with the sustained intracellular delivery of dexamethasone from microfluidics-synthesized nanoparticles, we explored the effects of particle design on controlling stem cell fates. We believe that the insights learned from this work will lead to the discovery of new strategies to tune differentiation for in situ differentiation or stem cell therapeutics.

From the Clinical Editor

The use of mesenchymal stem cells has been described by many researchers as a novel therapy for bone regeneration. One major hurdle in this approach is the control of osteogenic differentiation. In this article, the authors described elegantly their microfluidic system in which dexamethasone loaded nanoparticles were produced. This system would allow precise fabrication of nanoparticles and consequently higher efficiency in cellular differentiation.