Validating multiplexes for use in conjunction with modern interpretation strategies

详细信息    查看全文

• 作者：Duncan Taylor^a ; ^b ; ^{Duncan.Taylor@sa.gov.au" class="auth_mail" title="E-mail the corresponding author} ; Jo-Anne Bright^c ; Catherine McGoven^c ; Christopher Hefford^a ; Tim Kalafut^d ; John Buckleton^c
• 关键词：Forensic DNA interpretation ; GlobalFiler ; Continuous DNA interpretation ; STRmix ; Modelling ; DNA mixtures
• 刊名：Forensic Science International: Genetics
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：20
• 期：Complete
• 页码：6-19
• 全文大小：888 K

文摘

In response to requests from the forensic community, commercial companies are generating larger, more sensitive, and more discriminating STR multiplexes. These multiplexes are now applied to a wider range of samples including complex multi-person mixtures. In parallel there is an overdue reappraisal of profile interpretation methodology. Aspects of this reappraisal include

1.

The need for a quantitative understanding of allele and stutter peak heights and their variability,

2.

An interest in reassessing the utility of smaller peaks below the often used analytical threshold,

3.

A need to understand not just the occurrence of peak drop-in but also the height distribution of such peaks, and

4.

A need to understand the limitations of the multiplex-interpretation strategy pair implemented.

In this work we present a full scheme for validation of a new multiplex that is suitable for informing modern interpretation practice. We predominantly use GlobalFiler™ as an example multiplex but we suggest that the aspects investigated here are fundamental to introducing any multiplex in the modern interpretation environment.