Comparison of cell-based and PCR-based assays as methods for measuring infectivity of Tulane virus
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- 作者：Lei Shana ; David Yanga ; Dapeng Wangb ; Peng Tiana ; peng.tian@ars.usda.gov" class="auth_mail" title="E-mail the corresponding author
- 关键词：Tulane virus ; Correlation ; Plaque assay ; TCID50 assay ; ISC-qRT-PCR assay ; RNase exposure assay ; Infectivity ; Correlation
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：231
- 期：Complete
- 页码：1-7
- 全文大小：425 K
文摘
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Cell-based and PCR-based assays for infectivity of Tulane virus were compared.
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A strong positive linear relationship was identified between PFU and TCID50.
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No strong positive linear relationship between cell-based and PCR-based assays.
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TCID50 assay is more sensitive than plaque assay.