The heat-shock protein 70 gene is a suitable molecular marker for studying Trypanosoma phylogeny.
Phylogeny based on hsp70 supports classification of Trypanosoma in clades sensu Simpson et al. (2006).
In the T. cruzi clade three groups are discriminated: TcI, TcIII, and TcII + V + VI, while TcIV is not well supported.