Cloning and functional characterization of TCRP1, a novel gene mediating resistance to cisplatin in an oral squamous cell carcinoma cell line

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• 作者：Yixue Gu^a ; ^d ; Shasha Fan^a ; Yan Xiong^b ; Bo Peng^a ; Guopei Zheng^a ; Yanhui Yu^a ; Yongmei Ouyang^a ; Zhimin He^a ; ^c ; ^{hezhimin2005@yahoo.com}
• 关键词：Gene cloning ; TCRP1 ; OSCC ; Cisplatin ; Apoptosis
• 刊名：FEBS Letters
• 出版年：2011
• 出版时间：23 March 2011
• 年：2011
• 卷：585
• 期：6
• 页码：881-887
• 全文大小：1011 K

文摘

To explore the mechanisms of chemotherapy resistance, we previously established a multi-drug resistant cell line, Tca8113/Pingyangmycin (Tca8113/PYM) and identified differential expression in known genes and ESTs using microarray analysis. From among those ESTs we have now identified a novel gene producing an mRNA of 1834 nucleotides translated into a protein having 235 amino acids. This gene was denominated as tongue cancer resistance-associated protein 1 gene (TCRP1, accession number: EF363480). We further determined its functional characteristics. The results demonstrate that TCRP1 mediates a specific resistance to cisplatin in Tca8113 cells by reducing the cisplatin-induced apoptosis. This suggests that TCRP1 might be a novel molecular target to develop agents to reverse cisplatin-induced chemoresistance.