Complete mitochondrial genome of Chinese big-headed turtle, Platysternon megacephalum, with a novel gene organization in vertebrate mtDNA

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• 作者：Qiao-Ling Peng ; Liu-Wang Nie and You-Guang Pu
• 关键词：Big-headed turtle ; Mitochondrial genome ; LA-PCR ; Rearrangement ; Non-coding region
• 刊名：Gene
• 出版年：2006
• 出版时间：15 September 2006
• 年：2006
• 卷：380
• 期：1
• 页码：14-20
• 全文大小：401 K

文摘

The mitochondrial genome of the Chinese big-headed turtle, Platysternon megacephalum, was obtained using polymerase chain reaction (PCR). The entire mtDNA sequence, the longest mitochondrial genome in turtles reported so far, is 19 161 bp. This mitochondrial genome exhibits a novel gene order, which greatly differs from that of any other vertebrates. It is characterized by four distinctive features: 1) the translocation of a gene cluster including three tRNA genes (tRNA^His, tRNA^Ser, tRNA^Leu(CUN)) and ND5 gene, 2) two tRNA^Thr pseudogenes, 3) a duplication of pseudo tRNA^Thr/tRNA^Pro/D-loop region and 4) 3 non-coding spacers. These unique identities represent a new mitogenomic gene order in vertebrates. The TDRL model was proposed to account for the generation of the gene order in P. megacephalum.