Relevance of substrates and products on the desorption of lipases physically adsorbed on hydrophobic supports
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- 作者：Jose J. Virgen-Ortí ; za ; b ; 1 ; Veymar G. Tacias-Pascacioa ; c ; 1 ; Daniela B. Hiratad ; Beatriz Torrestiana-Sanchezc ; Arnulfo Rosales-Quinteroe ; Roberto Fernandez-Lafuentea ; rfl@icp.csic.es" class="auth_mail" title="E-mail the corresponding author ; rfernandezlafuente@hotmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Lipase interfacial activation ; Octyl agarose ; Detergents ; Enzyme leakage ; Enzyme reuse
- 刊名：Enzyme and Microbial Technology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：96
- 期：Complete
- 页码：30-35
- 全文大小：990 K
文摘
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CALB was rapidly immobilized on octyl-agarose.
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Most enzyme molecules remained immobilized in 80% ethanol.
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Most enzyme molecules were released if adding triglycerides as substrates.
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Even in fully aqueous media some enzyme release was produced.