Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells

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• 作者：Sayaka Higuchi¹ ; Qingsong Lin² ; Jigang Wang² ; Teck Kwang Lim² ; Shashikant B. Joshi¹ ; Ganesh Srinivasan Anand² ; Maxey C.M. Chung² ; ³ ; Michael P. Sheetz¹ ; Hideaki Fujita¹ ; ⁴ ; ⁵ ; ^{hideaki.fujita@riken.jp}
• 关键词：Embryonic stem cells ; Cardiomyocyte ; Differentiation ; Extracellular matrix ; Mechanobiology
• 刊名：Journal of Bioscience and Bioengineering
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：115
• 期：3
• 页码：320-325
• 全文大小：1575 K

文摘

We have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation, but role of ECM possessing a 3D structure in differentiation has not been studied in detail. We found that there are substantial differences in the quantitative protein profiles of ECM in SDS-treated heart tissue compared to that of liver tissue, as assessed by iTRAQ? quantitative proteomics analysis. When mouse ES cells were cultured on thin (60?¦Ìm) sections of de-cellularized tissue, the expression of cardiac myosin heavy chain (cMHC) and cardiac troponin I (cTnI) was high in ES cells cultured on heart ECM compared with those cultured on liver ECM. In addition, the protein expression of cMHC and cTnI was detected in cells on heart ECM after 2 weeks, which was not detectable in cells on liver ECM. These results indicate that heart ECM plays a critical role in the cardiomyocyte differentiation of ES cells. We propose that tissue-specific ECM induced cell lineage specification through mechano-transduction mediated by the structure, elasticity and components of ECM.