α-Cyclodextrin enhances myoblast fusion and muscle differentiation by the release of IL-4

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• 作者：Ana Claudia Batista Possidonio^a ; ¹ ; Mariana Lopes Senna^a ; ¹ ; Debora Morueco Portilho^a ; Carolina Pontes Soares^a ; Luzia da Silva Sampaio^b ; Marcelo Einicker-Lamas^b ; Morgana Teixeira Lima Castelo Branco^a ; Manoel Luis Costa^a ; Claudia Mermelstein^a ; ^{mermelstein@ufrj.br"" rel=""nofollow}
• 关键词：α ; -Cyclodextrin ; Methyl-β ; -cyclodextrin ; Myoblast fusion ; IL-4 ; Myogenin
• 刊名：Cytokine
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：55
• 期：2
• 页码：280-287
• 全文大小：1734 K

文摘

Muscle fibers are formed during embryonic development by the fusion of mononucleated myoblasts. The spatial structure and molecular composition of the sarcolemma are crucial for the myoblast recognition and fusion steps. Cyclodextrins are a group of substances that have the ability to solubilize lipids through the formation of molecular inclusion complexes. Previously, we have shown that methyl-β-cyclodextrin (MbCD) enhances muscle differentiation. Here, we analyzed the effects of α-cyclodextrin (aCD) during myogenesis. Myogenic cultures treated with aCD showed an increase in myoblast fusion and in the expression of myogenin, sarcomeric tropomyosin and desmin. aCD-conditioned media accelerates myogenesis in a similar way as aCD does, and increased levels of IL-4 were found in aCD-conditioned media. aCD-induced effects on myogenesis were inhibited by an anti-IL4 antibody. These results show that α-cyclodextrin induces myogenic differentiation by the release of IL-4.