Protective effect of atorvastatin on bone tissue in orchidectomised male albino Wistar rats

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• 作者：Iveta Gradosova^a ; ^{gradosovai@lfhk.cuni.cz} ; Helena Zivna^b ; ^{zivna@lfhk.cuni.cz} ; Vladimir Palicka^a ; ^{palicka@lfhk.cuni.cz} ; Sona Hubena^a ; ^{sona.hubena@lfhk.cuni.cz} ; Klara Svejkovska^a ; ^{svejkovskak@lfhk.cuni.cz} ; Pavel Zivny^a ; ^{zivny@lfhk.cuni.cz}
• 关键词：Atorvastatin ; Orchidectomised rat ; Bone marker ; Bone mineral density ; Biomechanical testing
• 刊名：European Journal of Pharmacology
• 出版年：2012
• 出版时间：15 March, 2012
• 年：2012
• 卷：679
• 期：1-3
• 页码：144-150
• 全文大小：611 K

文摘

Recent studies have shown that atorvastatin influences bone metabolism. We investigated its bone protective effect in orchidectomised rats after 12 weeks of treatment. Eight-week-old rats were divided into 3 groups: sham-operated group, control group after orchidectomy and experimental group after orchidectomy with atorvastatin administration (12 mg/kg/day). Bone mineral density and bone marker concentrations of aminoterminal propeptide of procollagen type I (PINP), osteoprotegerin (OPG), insulin-like growth factor 1 (IGF-1) in serum, and carboxy-terminal cross-linking telopeptide of type I collagen (CTX-I), bone alkaline phosphatase (BALP), bone morphogenetic protein 2 (BMP-2) in bone homogenate were measured. Total serum calcium and tibial calcium content was determined. Femurs were used for three-point bending test of the shaft and compression testing of the femoral neck. Bone markers (CTX-I, BALP, BMP-2) in control rats were higher vs. sham-operated rats. Atorvastatin reduced CTX-I, BMP-2 and OPG compared to controls. IGF-1 was decreased in control rats vs. sham-operated rats; atorvastatin increased IGF-1 vs. control rats. Atorvastatin exerts a positive effect on bone metabolism by increasing bone mineral density of the whole body, which had decreased under the effects of orchidectomy. Three-point bending test revealed an increase in maximal load values of the left femurs after atorvastatin administration compared to controls. The diameter of the left femur and length of both femurs were increased after atorvastatin administration compared to controls. Our findings suggest that atorvastatin has a beneficial effect on bone metabolism in orchidectomised rats by decreasing bone turnover, with resulting improvement in bone mineral density and bone biomechanical properties.