Mesenchymal stromal cells from bone marrow treated with bovine tendon extract acquire the phenotype of mature tenocytes
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- 作者：Lí ; via Maria Mendonç ; a Augusto ; liviiaaugusto@gmail.com" class="auth_mail" title="E-mail the corresponding author ; livia_augusto@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Diego Pinheiro Aguiar ; Danielle Cabral Bonfim ; Amanda dos Santos Cavalcanti ; Priscila Ladeira Casado ; Maria Eugê ; nia Leite Duarte
- 关键词：Tendon ; Mesenchymal stromal cells from bone marrow ; Tenocytes
- 刊名：Revista Brasileira de Ortopedia (English Edition)
- 出版年：2016
- 出版时间：January-February 2016
- 年：2016
- 卷：51
- 期：1
- 页码：70-74
- 全文大小：699 K
文摘
This study evaluated in vitro differentiation of mesenchymal stromal cells isolated from bone marrow, in tenocytes after treatment with bovine tendon extract.
Methods
Bovine tendons were used for preparation of the extract and were stored at −80 °C. Mesenchymal stromal cells from the bone marrow of three donors were used for cytotoxicity tests by means of MTT and cell differentiation by means of qPCR.
Results
The data showed that mesenchymal stromal cells from bone marrow treated for up to 21 days in the presence of bovine tendon extract diluted at diminishing concentrations (1:10, 1:50 and 1:250) promoted activation of biglycan, collagen type I and fibromodulin expression.
Conclusion
Our results show that bovine tendon extract is capable of promoting differentiation of bone marrow stromal cells in tenocytes.