Ethanol fermentation by xylose-assimilating Saccharomyces cerevisiae using sugars in a rice straw liquid hydrolysate concentrated by nanofiltration

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• 作者：Kengo Sasaki ; Daisuke Sasaki ; Yuri Sakihama ; Hiroshi Teramura ; Ryosuke Yamada ; Tomohisa Hasunuma ; Chiaki Ogino ; Akihiko Kondo
• 关键词：Rice straw hydrolysate ; Nanofiltration ; Glucose ; Xylose ; Ethanol fermentation
• 刊名：Bioresource Technology
• 出版年：2013
• 出版时间：November, 2013
• 年：2013
• 卷：147
• 期：Complete
• 页码：84-88
• 全文大小：509 K

文摘

Concentrating sugars using membrane separation, followed by ethanol fermentation by recombinant xylose-assimilating Saccharomyces cerevisiae, is an attractive technology. Three nanofiltration membranes (NTR-729HF, NTR-7250, and ESNA3) were effective in concentrating glucose, fructose, and sucrose from dilute molasses solution and no permeation of sucrose. The separation factors of acetate, formate, furfural, and 5-hydroxymethyl furfural, which were produced by dilute acid pretreatment of rice straw, over glucose after passage through these three membranes were 3.37-11.22, 4.71-20.27, 4.32-16.45, and 4.05-16.84, respectively, at pH 5.0, an applied pressure of 1.5 or 2.0 MPa, and 25 ¡ãC. The separation factors of these fermentation inhibitors over xylose were infinite, as there was no permeation of xylose. Ethanol production from approximately two-times concentrated liquid hydrolysate using recombinant S. cerevisiae was double (5.34-6.44 g L^-1) that compared with fermentation of liquid hydrolysate before membrane separation (2.75 g L^-1).