Development of a multi-class method for the quantification of veterinary drug residues in feedingstuffs by liquid chromatography-tandem mass spectrometry

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• 作者：Aurore Boscher ; C&#xe9 ; dric Guignard ; Terence Pellet ; Lucien Hoffmann ; Torsten Bohn
• 关键词：Antibiotics ; Coccidiostats ; LC– ; MS/MS ; Feed ; Standard addition method ; Matrix effects
• 刊名：Journal of Chromatography A
• 出版年：2010
• 出版时间：8 October 2010
• 年：2010
• 卷：1217
• 期：41
• 页码：6394-6404
• 全文大小：646 K

文摘

A simple multi-residue method was developed for detecting and quantifying 33 analytes from 13 classes of antibiotics (tetracyclines (3), quinolones (7), penicillins (3), ionophore coccidiostats (7), macrolides (3), sulfonamides (1), quinoxalines (2), phenicols (2), lincosamides (1), diaminopyrimidines (1), polypeptides (1), streptogramins (1) and pleuromutilins (1)) in animal feeds. Extraction and clean-up procedures were optimized with spiked piglet feed. Samples were extracted by ultrasonic-assisted extraction with a mixture of methanol/acetonitrile/McIlvaine buffer at pH 4.6 (37.5/37.5/25, v/v/v) containing 0.3 % of EDTA-Na₂, followed by a clean up using a dispersive solid-phase extraction (d-SPE) with PSA (primary secondary amine). Detection of antibiotics was achieved by liquid chromatography-electrospray tandem mass spectrometry (LC–ESI-MS/MS) within 28 min using both positive and negative ESI mode. Average recoveries ranged from 51 % (oxytetracycline) to 116 % (tilmicosin) with associated relative standard deviations of 7.3 % and 9.0 % and an overall mean of 87 % . Limits of quantification ranged from 3.8 ng g⁻¹ (lincomycin) to 65.0 ng g⁻¹ (bacitracin). Following optimization, the method was further verified for bovine and lamb feedingstuffs; negative matrix effects were evaluated and overcome by a standard addition method.