Characterization of the rabbit agouti signaling protein (ASIP) gene: Transcripts and phylogenetic analyses and identification of the causative mutation of the nonagouti black coat colour

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• 作者：Luca Fontanesi ; Lionel Forestier ; Daniel Allain ; Emilio Scotti ; Francesca Beretti ; S&#xe9 ; verine Deretz-Picoulet ; Elena Pecchioli ; Cristiano Vernesi ; Terence J. Robinson ; Jason L. Malaney ; Vincenzo Ru
• 关键词：Agouti ; Coat colour ; Lagomorpha ; Mutation ; Oryctolagus cuniculus ; Rabbit ; Transcription analysis
• 刊名：Genomics
• 出版年：2010
• 出版时间：March 2010
• 年：2010
• 卷：95
• 期：3
• 页码：166-175
• 全文大小：1170 K

文摘

The agouti locus encodes the agouti signalling protein (ASIP) which is involved in determining the switch from eumelanin to pheomelanin synthesis in melanocytes. In the domestic rabbit (Oryctolagus cuniculus) early studies indicated three alleles at this locus: A, light-bellied agouti (wild type); a^t, black and tan; a, black nonagouti. We characterized the rabbit ASIP gene and identified the causative mutation (an insertion in exon 2) of the black nonagouti allele whose frequency was evaluated in 31 breeds. Phylogenetic analysis of ASIP sequences from Oryctolagus and 9 other species of the family Leporidae placed Oryctolagus as sister species to Pentalagus and Bunolagus. Transcription analysis in wild type agouti rabbits revealed the presence of two major transcripts with different 5′-untranslated regions having ventral or dorsal skin specific expression. ASIP gene transcripts were also detected in all examined rabbit tissues distinguishing the rabbit expression pattern from what was observed in wild type mice.