The upstream regulatory sequence of the light harvesting complex Lhcf2 gene of the marine diatom Phaeodactylum tricornutum enhances transcription in an orientation- and distance-independent fashion

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• 作者：Monia Teresa Russo^a ; ^{monia.russo@szn.it" class="auth_mail" title="E-mail the corresponding author} ; Rossella Annunziata^b ; ^c ; Remo Sanges^a ; Maria Immacolata Ferrante^a ; Angela Falciatore^b ; ^c ; ^{angela.falciatore@upmc.fr" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Diatoms ; Gene expression ; Promoter ; Enhancer ; Regulatory element
• 刊名：Marine Genomics
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：24
• 期：part_P1
• 页码：69-79
• 全文大小：1059 K

文摘

Diatoms are a key phytoplankton group in the contemporary ocean, showing extraordinary adaptation capacities to rapidly changing environments. The recent availability of whole genome sequences from representative species has revealed distinct features in their genomes, like novel combinations of genes encoding distinct metabolisms and a significant number of diatom-specific genes. However, the regulatory mechanisms driving diatom gene expression are still largely uncharacterized. Considering the wide variety of fields of study orbiting diatoms, ranging from ecology, evolutionary biology to biotechnology, it is thus essential to increase our understanding of fundamental gene regulatory processes such as transcriptional regulation. To this aim, we explored the functional properties of the 5′-flanking region of the Phaeodatylum tricornutum Lhcf2 gene, encoding a member of the Light Harvesting Complex superfamily and we showed that this region enhances transcription of a GUS reporter gene in an orientation- and distance-independent fashion. This represents the first example of a cis-regulatory sequence with enhancer-like features discovered in diatoms and it is instrumental for the generation of novel genetic tools and diatom exploitation in different areas of study.