Identification of methicillin-resistant isolates of Staphylococcus aureus and coagulase-negative staphylococci responsible for bloodstream infections with the Phoenix™ system

详细信息    查看全文

• 作者：Spanu ; Teresa ; Sanguinetti ; Maurizio ; D'Inzeo ; Tiziana ; Ciccaglione ; Daniela ; Romano ; Lucio ; et. al.
• 关键词：Methicillin resistance ; Staphylococcal bloodstream isolates
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2004
• 出版时间：April, 2004
• 年：2004
• 卷：48
• 期：4
• 页码：221-227
• 全文大小：92.5 K

文摘

We evaluated the reliability of the new Phoenix™ system (Becton Dickinson Microbiology Systems, Sparks, Md.) in species-level identification and detection of oxacillin (methicillin) resistance among 493 staphylococcal isolates (Staphylococcus aureus, n = 223; coagulase-negative staphylococci, CoNS, n = 270) recovered from patients with bacteremia. Identification results were concordant with those of the ID 32 STAPH system (bioMérieux, Marcy l'Etoile, France) for 100 % of S. aureus (223/223) and 97.4 % (263/270) of CoNS isolates. For S. aureus isolates, Phoenix oxacillin-susceptibility results fully concurred with those of mecA polymerase chain reaction (PCR) (reference method): 96 mecA-positive isolates identified as resistant, 127 mecA-negative strains as susceptible. Two of the 210 mecA-positive CoNS isolates were misclassified as susceptible by the Phoenix™ (sensitivity 99 % , positive predictive value 97.6 % ). Five of 60 mecA-negative CoNS isolates were classified as resistant by the Phoenix™ (specificity 91.7 % ; negative predictive value 96.5 % ). The Phoenix™ system can provide accurate and reliable identification of methicillin-resistant staphylococci responsible for bloodstream infections.