Consequences of tributyltin chloride induced stress in Leydig cells: An ex-vivo approach

详细信息    查看全文

• 作者：Sumonto Mitra ; Ankit Srivastava ; Smita Khanna ; Shashi Khandelwal ; ^{shashik.iitr@gmail.com" class="auth_mail} ; ^{msumonto@gmail.com" class="auth_mail}
• 关键词：ROS ; reactive oxygen species ; GSH ; glutathione ; TBARS ; thiobarbituric acid reactive substances ; BAPTA-AM ; (1 ; 2-bis(o-aminophenoxy)ethane-N ; N ; N&prime ; N&prime ; -tetraacetic acid) ; EGTA ; ethylene glycol tetraacetic acid ; NAC ; N-acetyl cysteine ; ERK1/2 ; extracellular signal-regulated kinase 1/2 ; JNK1/2 ; c-jun amino-terminal kinases 1/2 ; 3尾-HSD ; 3ß ; hydroxysteroid dehydrogenases ; StAR ; steroidogenic acute regulatory protein ; TSPO ; translocator protein 18  ; kDa ; PI ; propidium iodide
• 刊名：Environmental Toxicology and Pharmacology
• 出版年：March, 2014
• 年：2014
• 卷：37
• 期：2
• 页码：850-860
• 全文大小：2182 K

文摘

Tributyltin (TBT), a member of the organotin family, is a known endocrine disruptor. It persists long in the environment and is widely used in various industrial applications. This study was planned to understand its toxic influence on Leydig cells isolated from 28 day old wistar rats. In-vitro exposure to TBT-Chloride (TBTC) (300-3000 nM) reduced cell viability (DNA fragmentation, nuclear condensation and MTT assay) and affected testosterone production. TBTC induced both apoptotic and necrotic cell death (Annexin V/PI binding assay). Involvement of calcium (Ca²⁺), redox imbalance (ROS, GSH and TBARS) and mitochondria in TBTC toxicity was evaluated by using Ca²⁺ inhibitors (BAPTA-AM, EGTA, Ruthenium Red), free radical scavengers (NAC, C-Phycocyanin) and mitochondrial permeability transition pore inhibitor (Cyclosporine A). Protein expression analysis of phosphorylated MAPKinases (ERK1/2, JNK1/2, & p38), steroidogenic proteins (3尾-HSD, StAR & TSPO) and apoptotic proteins (Bax, Bcl2) illustrates the cytotoxic and anti-steroidogenic activity of TBTC.