Topogenesis and cell surface trafficking of GPR34 are facilitated by positive-inside rule that effects through a tri-basic motif in the first intracellular loop
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- 作者：Haruki Hasegawa ; harukih@amgen.com" class="auth_mail" title="E-mail the corresponding author ; h.haruki@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Neha Patel ; Elham Ettehadieh ; Peng Li ; Ai Ching Lim
- 关键词：DIC ; differential interference contrast ; ER ; endoplasmic reticulum ; GPCR ; G protein-coupled receptor ; HEK ; human embryonic kidney ; TCEP ; tris(2-carboxyethyl)phosphine
- 刊名：Biochimica et Biophysica Acta (BBA)-Molecular Cell Research
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：1863
- 期：7
- 页码：1534-1551
- 全文大小：3869 K
文摘
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GPR34 topogenesis and cell surface trafficking are regulated by positive-inside rule.
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A tri-basic motif in the first intracellular loop is the key topogenic signal.
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Disruption of the motif led to erroneous topology and trafficking arrest in Golgi.
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Exogenous N-terminal signal sequence rectified the topology and trafficking.
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Biochemical properties of the key native conformational epitope were elucidated.