Heterologous expression and characterization of CYP61A1 from dandruff-causing Malassezia globosa
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- 作者：Seul-Ong Ohka ; Hyoung-Goo Parkb ; Hwayoun Leeb ; Yeo-Jung Kwona ; Beom Joon Kimc ; Donghak Kimb ; donghak@konkuk.ac.kr" class="auth_mail" title="E-mail the corresponding author ; Young-Jin Chuna ; yjchun@cau.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：CYP (or P450) ; cytochrome P450 ; PCR ; polymerase chain reaction ; TB ; terrific broth ; DTT ; dithiothreitol
- 刊名：Protein Expression and Purification
- 出版年：2015
- 出版时间：October 2015
- 年：2015
- 卷：114
- 期：Complete
- 页码：89-94
- 全文大小：812 K
文摘
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CYP61A1 is a sterol C-22 desaturase in dandruff-causing M. globosa.
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Recombinant CYP61A1 was heterologously expressed and purified.
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CYP61A1 demonstrated the type I binding spectra with 尾-sitosterol and campesterol.
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The tight binding to antifungal azoles suggests that CYP61A1may be a therapeutic target.
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Study of CYP61A1 leads to a better understanding P450 for fungal sterols biosynthesis.