Selective retrieval of microparticles in microchambers using electrolytically generated bubbles for cell array applications

详细信息    查看全文

• 作者：Tomoaki  ; Kurakazu ; Kaori  ; Kuribayashi-Shigetomi ; Yukiko T.  ; Matsunaga ; Hiroshi  ; Kimura ; Teruo  ; Fujii ; Yasuyuki  ; Sakai ; Shoji  ; Takeuchi  ; ;   ; ^{takeuchi@iis.u-tokyo.ac.jp}[Author vitae]
• 关键词：Selective retrieval ; Bubble ; Electrolysis ; Microfluidic device ; Cell manipulation
• 刊名：Sensors and Actuators B: Chemical
• 出版年：2011
• 出版时间：28 November 2011
• 年：2011
• 卷：159
• 期：1
• 页码：229-233
• 全文大小：782 K

文摘

This paper describes a method for the selective retrieval of microparticles using bubbles generated by electrolysis. Microparticles (polystyrene beads, mouse embryos, and cell spheroids) were arrayed in microchambers fabricated in SU-8 on the surface of an electrode consisting of indium tin oxide (ITO) patterned on glass. Bubbles were selectively generated in a target microchamber by applying a voltage to electrodes positioned in the microchamber. As a result, we successfully retrieved microparticles (100 ¦Ìm in diameter) positioned in the microchambers. This method is gentle enough to maintain cellular viability, and therefore, it will be a powerful tool for the quantitative analysis of cells in an arrayed system.