Current status of extended spectrum β-lactamase-producing Escherichia coli, Klebsiella pneumoniae and Proteus mirabilis in Okinawa prefecture, Japan

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• 作者：Rika Nakama^a ; Aoi Shingaki^a ; Hiroko Miyazato^b ; Rikako Higa^a ; Chota Nagamoto^a ; Kouta Hamamoto^a ; Shuhei Ueda^a ; ^c ; Teruyuki Hachiman^b ; ^d ; Yuki Touma^a ; Kazufumi Miyagi^a ; Ryuji Kawahara^c ; ^e ; Takehiko Toyosato^f ; Itaru Hirai^a ; ^c ; ^{hiraii@med.u-ryukyu.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CTX-M-type extended spectrum β-lactamase ; Klebsiella pneumoniae possessing blaSHV ; Prevalence
• 刊名：Journal of Infection and Chemotherapy
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：22
• 期：5
• 页码：281-286
• 全文大小：387 K

文摘

Enterobacteriaceae producing extended spectrum β-lactamase (ESBL) are distributed worldwide. In this study, 114 ESBL-producing Enterobacteriaceae were isolated by analyzing 1672 clinical isolates of Enterobacteriaceae collected from an Okinawa prefectural hospital in Japan between June 2013 and July 2014. The overall prevalence of ESBL-producing Enterobacteriaceae was 6.8%; the prevalence of different bacterial species among the ESBL-producing isolates was as follows: 11.5% Escherichia coli (90 of 783 isolates), 6.2% Klebsiella pneumoniae (19 of 307 isolates), and 11.1% Proteus mirabilis (5 of 45 isolates). The ESBL types bla_CTX-M-1, _-3, _-15, _-2, _-14, _-27, and mutants of bla_SHV-1 were detected. Among them, bla_CTX-M-15 (33.3%), bla_CTX-M-14 (27.8%) and bla_CTX-M-27 (33.3%) were dominant in the E. coli isolates, whereas a bla_SHV mutant which possessed four mutations (Tyr7Phe, Leu35Gln, Gly238Ser and Glu240Lys) in the amino acid sequence of SHV-1 dominated in the K. pneumoniae isolates (11 of 19, 57.9%). The pandemic E. coli ST131 clone was found to constitute 3.3% of the overall examined isolates and 62.2% of the ESBL-producing E. coli isolates. Our results suggest that the genetic combination of bla_CTX-M, and bla_SHV and antibiotics-resistant profile were different from that in other regions such as other areas of Japan, Asia, Europe, and North America, especially in the ESBL-producing K. pneumoniae isolates and in the E. coli B2-O25b-ST131 isolates possessing bla_CTX-M-15 (40.7% of the E. coli B2-O25b-ST131 isolates). Taken together, our results indicate that the ESBL-producing Enterobacteriaceae in Okinawa, Japan, might be of a unique nature.