Phylogenetic analysis of nuclear small subunit rDNA sequences suggests that the endangered African Pencil Cedar, Juniperus procera, is associated with distinct members of Glomeraceae

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• 作者：Tesfaye WUBET ; Michael WEIβ ; Ingrid KOTTKE ; Demel TEKETAY ; Franz OBERWINKLER
• 关键词：African Pencil Cedar ; Arbuscular mycorrhizal (AM) fungi ; Dry Afromontane forest ; Ethiopia ; Juniperus procera ; nuSSU rDNA
• 刊名：Mycological Research
• 出版年：2006
• 出版时间：September 2006
• 年：2006
• 卷：110
• 期：9
• 页码：1059-1069
• 全文大小：360 K

文摘

The endangered indigenous tree species Juniperus procera, commonly known as African Pencil Cedar, is an important component of the dry Afromontane vegetation of Ethiopia and was shown to be AM in earlier studies. Here we describe the composition of AM fungi in colonized roots of J. procera from two dry Afromontane forests of Ethiopia. The nuSSU rDNA gene was amplified from colonized roots, cloned and sequenced using AM fungal specific primers that were partly developed for this study. Molecular phylogenetic analysis revealed that all the glomeralean sequences obtained belonged exclusively to the genus Glomus (Glomeraceae). Seven distinct Glomus sequence types were identified that all are new to science. The composition of the AM fungal communities between the sampled trees, and between the two study sites in general, differed significantly. Isolation and utilization of the indigenous AM fungal taxa from the respective sites might be required for successful enrichment plantation of this threatened Juniperus species.