Scheduled multiple reaction monitoring algorithm as a way to analyse new designer drugs combined with synthetic cannabinoids in human serum with liquid chromatography-tandem mass spectrometry

详细信息    查看全文

• 作者：Marek Dziadosz ; ^{Dziadosz.Marek@mh-hannover.de} ; ^{analytiker@chemist.com} ; Jens-Peter Weller ; Michael Klintschar ; Jö ; rg Teske
• 关键词：Synthetic cannabinoids ; Cathinones ; Piperazines ; Amphetamines ; Pyrovalerones ; Scheduled MRM
• 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
• 出版年：2013
• 出版时间：15 June, 2013
• 年：2013
• 卷：929
• 期：Complete
• 页码：84-89
• 全文大小：669 K

文摘

Here, we describe the development and application of a liquid chromatography-tandem mass spectrometry method with positive electrospray ionisation and scheduled multiple reaction monitoring algorithm (s-MRM) to analyse synthetic cannabinoids (SC) combined with new designer drugs (NDD) in human serum. A Luna 5 ¦Ìm C18 (2) 100 A, 150 mm ¡Á 2 mm analytical column and a mobile phase consisted of A (H₂O/methanol = 95/5, v/v) and B (H₂O/methanol = 3/97, v/v) - both with 10 mM ammonium acetate and 0.1 % acetic acid (pH = 3.2), were used for the separation. A binary flow pumping mode with a total flow rate of 0.400 mL/min was used. A single sample extraction with 1-chlorobutane for both substance groups was performed. Acceptable linearity in the validated calibration ranges of 0.05-1 ng/mL for SC and 1-50 ng/mL for NDD was achieved. The limit of detection was not greater than 0.02/0.40 ng/mL and the limit of quantification not greater than 0.05/0.50 ng/mL for SC/NDD respectively. The presented study revealed that this method is a very effective way for sensitive SC and NDD identification in human serum and has useful application in hospitals, therapy centres and forensic psychiatric centres. S-MRM ensures a method upgrade with a smaller loss of sensitivity, precision and accuracy in comparison to traditional MRM methods. Also addition of new SC and NDD can be performed in the future.