Standardization of a well-controlled in vivo mouse model of thrombus formation induced by mechanical injury
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- 作者：Chaojun Tanga ; 1 ; zjtang@suda.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Yinyan Wanga ; 1 ; Daoxi Leib ; Lu Huangb ; Guixue Wangb ; Qingjia Chib ; Yiming Zhengb ; Christian Gachetc ; Pierre H. Manginc ; Li Zhua
- 关键词：DIOC6 ; 3 ; 3&prime ; -dihexyloxacarbocyanine iodide ; CCD ; Charge Coupled Device ; AUC ; area under curve ; SEM ; scanning electron microscopy ; FeCl3 ; ferric chloride ; MRS 2500 ; (1R* ; 2S*)-4-[2-iodo-6-(methylamino) ; 9H-purin-9-yl]-2-(phosphonooxy) bicyclo [3.1.0] hexa ; ne-1-methanol dihydrogen phosphate ester tetraammonium salt
- 刊名：Thrombosis Research
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：141
- 期：Complete
- 页码：49-57
- 全文大小：1855 K
文摘
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This study aimed to standardize our previously established mouse model of thrombus formation induced by mechanical injury.
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We improved it by applying two degrees of calibrated pinching in order to induce two defined degrees of vessel injury severity.
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We analyzed the real-time thrombus formation, pathological changes of the vessel wall, and its applicability for anti-thrombotic evaluation.
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This model generates a large scope of vessel injury, allowing relatively flexible applications with reproducibility and feasibility.