Using patient serum to epitope map soybean glycinins reveals common epitopes shared with many legumes and tree nuts
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- 作者：Hanaa Saeed ; Christine Gagnon ; Elroy Cober ; Steve Gleddie ; steve.gleddie@agr.gc.ca" class="auth_mail" title="E-mail the corresponding author
- 关键词：AA ; amino acid ; DMSO ; dimethyl sulfoxide ; ELISA ; enzyme-linked immunosorbent assay ; HS ; hot spot ; IgE ; immunoglobulin E ; RAST ; radioallergosorbent test ; SDAP ; structural database of allergenic proteins ; SDS ; sodium dodecyl sulfate ; TBS-BSA 2% ; 2% bovine serum albumin in tris buffered saline ; TMB ; Tetramethylbenzidine
- 刊名：Molecular Immunology
- 出版年：2016
- 出版时间：February 2016
- 年：2016
- 卷：70
- 期：Complete
- 页码：125-133
- 全文大小：3229 K
文摘
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The IgE-binding B-cell epitopes of two similar soybean glycinin subunits (Gly m 6) are described.
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Three-dimensional modeling of the glycinin hexamers located most epitopes on the exposed surfaces.
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Comparison with other plant 11S storage protein sequences revealed one dominant and several shared epitopes.
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Common epitope hot spots are often shared between plant storage proteins from diverse families.